R5509-42 データシートの表示（PDF） - Hamamatsu Photonics
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R5509-42 Datasheet PDF : 12 Pages
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APPLICATION EXAMPLES
Measurement of singlet oxygen
Sample 11
Singlet oxygen
Rose Bengal in pure water
Using the R5509-42 and a pulsed las-
er, singlet oxygen emission with a
peak at 1270 nm were efficiently de-
tected by signal processing with a ga-
ted pulse counter, reducing effects of
fluorescence.
(Data obtained by CW YAG laser ex-
citation is also shown in the same
graph for comparison.)
The graph on the right shows detec-
tion limits evaluated by changing the
concentration of the photosensitizer
Rose Bengal. This proves that emis-
sions from singlet oxygen of low con-
centration, even only 1 nmol/L, can be
SAMPLE TEMPERATURE
300K
( ) room
temperature
2
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
1150
SAMPLE TEMPERATURE
300K
105
( ) room
temperature
104
103
GATED PHOTON COUNTING METHOD
EXCITATION LIGHT: PULSE SHG Nd: YAG (532 nm)
12 mJ, PULSE WIDTH: 10 ns, 20 Hz
SLIT: 2 mm/ 2 mm
GATED DELAY TIME: 1.5 µs
GATE TIME: 5 µs
CW
GATED PHOTON COUNTING METHOD
1200
1250
1300
WAVELENGTH (nm)
1350
TPMHB0665EA
EXCITATION LIGHT: PULSE SHG Nd: YAG (532 nm)
12 mJ, PULSE WIDTH: 10 ns, 20 Hz
SLIT: 2 mm / 2 mm
CONCENTRATION
OF ROSE BENGAL
10 µmol/L
1 µmol/L
1 nmol/L
102
Data was measured with a near infrared
measurement system described later.
Sample 12
Singlet oxygen
Rose Bengal in acetone, methanol and water
Lifetime characteristics and emission
spectrum of the singlet oxygen when
the photosensitizer Rose Bengal was
dissolved in acetone, methanol and
water were measured.
Singlet oxygen lifetime can be meas-
ured with high accuracy, by using ga-
ted photon counting techniques that
utilize high-speed response of a near
infrared PMT and allow continuous
scan of signal pulses obtained in a
short gate time (sampling time).
In solvents which singlet oxygen has a
long life, there is little singlet oxygen
that thermally disappears so more sin-
glet oxygen disappears during the
emission process. This results in an
increase in the entire emission level.
Data was measured with a near infrared
measurement system described later.
101
1100
1150
1200
1250
1300
1350
1400
1450
WAVELENGTH (nm)
TPMHB0666EA
SAMPLE TEMPERATURE
300K
104
( ) room
temperature
103
102
101
EXCITATION LIGHT: PULSE SHG Nd: YAG (532 nm)
2.5 mJ, PULSE WIDTH: 10 ns, 20 Hz
SLIT: 2 mm / 2 mm
GATE (sampling) TIME: 1 µs
τ=62 µs in CH3COCH3
(ACETONE)
τ=11 µs in CH3OH
(METHANOL)
τ=3.7 µs in H2O
(WATER)
100
0
10 20 30 40
50 60 70 80 90 100
SAMPLE TEMPERATURE
300K
1.4 × 104
( ) room
temperature
1.2 × 104
1.0 × 104
0.8 × 104
0.6 × 104
0.4 × 104
TIME (µs)
TPMHB0667EA
EXCITATION LIGHT:
PULSE SHG Nd:
YAG (532 nm)
2.5 mJ,
PULSE WIDTH:
10 ns, 20 Hz
SLIT: 2 mm / 2 mm
GATED DELAY TIME:
3 µs
GATE TIME: 50 µs
CH3COCH3
CH3OH
H2O
0.2 × 104
0
1200 1220 1240 1260 1280 1300 1320 1340
WAVELENGTH (nm)
TPMHB0668EA
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